Ngongo Whakamātautau Toto Iti 0.25ml 0.5ml 1ml

He hoahoa tangata te ngongo kohi toto moroiti, ā, he taupoki haumaru kua hiritia, ka taea e te ngongo te ārai i te turuturu toto. Nā te maha o ngā niho me te hanganga takirua, he watea mō te kawe haumaru me te whakahaere ngāwari, kāore he toto e maringi.

He rite te tae o te potae haumaru ki te Paerewa o te Ao, he māmā ki te tautuhi.

He tino ātaahua te hoahoa o te taha o te waha o te ngongo, ā, he māmā noa iho mō ngā kaiwhakamahi e totohu ana ki roto i te ngongo. He māmā, he tere, he mārama hoki te pānui i te nui o te toto me te rārangi tohu mārama.

He maimoatanga motuhake kei roto i te ngongo, he maeneene te mata, kāore he toto e piri.

Ka taea te whakarite i te waehere pae, me te whakahoromata i te ngongo ki ngā hihi gamma kia rite ki ngā hiahia o ngā kiritaki kia tutuki ai te whakamātautau asepsis.

1. Ngongo Whakaoho Kiri me te Totoka

Ka whakamahia te ngongo whakahohe ira me te toto totoka mō te matū matū toto, te ārai mate me te whakamātautau tarukino, me ētahi atu. Ka rehuhia te matū whakapūkara ki runga i te mata o roto i te ngongo, ka whakapoto i te wā totoka.

Nā te mea he matū parakore te tiēre wehewehe kawemai mai i Hapani, he tino pumau tōna āhuatanga ā-tinana, ka taea e ia te tu atu i ngā pāmahana tiketike kia mau tonu ai te pumau o te tiēre i te wā e rokiroki ana, e kawe ana hoki.

Ka totoka te tiēre i muri i te whakatōtōtanga, ā, ka wehea rawatia te toto mai i ngā pūtau fibrin, pērā i te ārai, e ārai ana i te whakawhitiwhiti matū i waenga i te toto me ngā pūtau. Ka pai ake te whai huatanga o te kohikohi toto, ā, ka whiwhihia he toto kounga teitei, nō reira ka puta he hua whakamātautau pono ake.

Kia mau tonu te pumau o te serum mō ngā haora neke atu i te 48, kāore he panonitanga kitea e pā ana ki ōna āhuatanga matū koiora me ngā hanganga matū, kātahi ka taea te whakamahi tika i te ngongo ki ngā tātaritanga tauira.

- Te wā mō te tango katoa i te toto totoka: 20-25 meneti
- Tere whakaterenga: 3500-4000r/m
- Te wā whakapūpū: 5 meneti
- Te pāmahana rokiroki e taunakitia ana: 4-25ºC

2. Ngongo Whakaoho Totoka

Ka whakamahia te ngongo whakaoho toto totoka i roto i te kohikohinga toto mō te matū koiora me te ārai mate i roto i te tirotiro hauora. He pai mō te whānuitanga o te pāmahana whakahaere. Mā te maimoatanga motuhake, he tino maeneene te mata o roto o te ngongo, ā, ka rehu ōrite te matū whakapūkara kounga teitei. Ka tino pa te tauira toto ki te matū whakapūkara ka totoka i roto i te 5-8 meneti. Nō reira, ka whiwhihia te serum kounga teitei mā te centrifugation i muri mai, kāore he pakaru o te pūtau toto, te hemolysis, te wehenga o te pūmua fibrin, me ētahi atu.

Nō reira, ka taea e te serum te whakatutuki i ngā whakaritenga o te whakamātautau serum tere i roto i te whare hauora me te whakamātautau serum ohorere.
- Te wā mō te tango katoa i te toto totoka: 20-25 meneti
- Tere whakaterenga: 3500-4000r/m
- Te wā whakapūpū: 5 meneti
- Te pāmahana rokiroki e taunakitia ana: 4-25ºC

3. Ngongo EDTA

E whakamahia whānuitia ana te ngongo EDTA i roto i te hematology haumanu, te whakataurite toto, te whakarōpūtanga toto me ngā momo taputapu whakamātautau pūtau toto.

He tiaki whānui tēnei mō ngā pūtau toto, inā koa mō te tiaki i ngā pereti toto, kia kore ai e kohia te pereti toto, kia kore ai hoki e pāngia te āhua me te rahinga o ngā pūtau toto i roto i te wā roa.

Ka taea e ngā kākahu tino pai me te tikanga tino poto te rehu i te tāpiringa kia ōrite ki te mata o roto o te ngongo, kia taea ai e te tauira toto te whakaranu katoa ki te tāpiringa. Ka whakamahia te plasma anticoagulant EDTA mō te whakamātautau koiora o ngā moroiti mate, ngā pirinoa me ngā ngota kitakita, me ētahi atu.

4. Ngongo DNA

1. Kua whakakīia te ngongo RNA/DNA toto ki te matū motuhake hei tiaki wawe i te RNA/DNA o ngā tauira kia kore ai e pirau.

2. Ka taea te rongoa i ngā tauira toto mō ngā rā e 3 i te 18-25°c, ka rongoa mō ngā rā e 5 i te 2-8°c, ka noho pumau mō te iti rawa 50 marama i te -20°c ki te -70°c

3. He māmā ki te whakamahi, hurihia noa te ngongo RNA/DNA toto i te 8 ngā wā i muri i te kohinga ka taea te whakaranu toto nui

4. Tāpirihia ki ngā toto hou o te tangata me ngā kararehe whakangote, kāore e pai mō ngā toto tawhito me ngā toto e pupū ana, tae atu ki ngā toto o ngā manu me ētahi atu kararehe.

5. Te kohikohinga, te rokiroki me te kawe paerewa o ngā tauira kimi RNA/DNA toto katoa

6. Ko te pakitara o roto o te ngongo he tukatuka motuhake me te kore RNase, DNase, kia mau tonu ai te hiranga o ngā tauira kimi waikawa karihi.

7. He pai ki te tango i ngā tauira me te tere o te tango, te whakapai ake i te whai huatanga o te taiwhanga

5. Ngongo ESR

Ko te Ngongo ESR Ø13×75mm e whakamahia ana mō te kohikohi toto me te ārai toto totoka mō te whakamātautau tere o te parahanga toto aunoa a te Kaitātari Tere Whakatakotoranga Erythrocyte me te ōwehenga whakaranu o te 1 wāhanga konutai citrate ki te 4 wāhanga toto, mā te tikanga Westergren.

6. Ngongo Huka

Ka whakamahia te ngongo hukahuka hei kohi toto mō ngā whakamātautau pēnei i te huka toto, te manawanui huka, te electrophoresis erythrocyte, te hemoglobin anti-alkali me te lactate. Mā te Sodium Fluoride i tāpirihia ka ārai i te pākia o te huka toto, ā, ka whakaoti angitu te Sodium Heparin i te hemolysis.

Nō reira, ka roa te noho o te toto ki te āhua taketake, ā, ka pumau tonu ngā raraunga whakamātautau mō te huka toto i roto i ngā haora 72. Ko te tāpiritanga kōwhiringa ko te Sodium Fluoride+Sodium Heparin, Sodium Fluoride+ EDTA.K2, Sodium Fluoride+EDTA.Na2.

Tere whakaterenga: 3500-4000 r/m
Te wā whakapūpū: 5min
Te pāmahana rokiroki e taunakitia ana: 4-25 ºC

7. Ngongo Heparin
Ka whakamahia te ngongo heparin hei kohikohi toto mō te whakamātautau i te plasma haumanu, te matū koiora ohorere me te rheology toto, me ētahi atu. He iti noa te whakararuraru ki ngā hanganga toto, ā, kāore he pānga ki te rahi o ngā erythrocyte, kāore e puta he hemolysis. Haunga tēnā, he tere te wehenga o te plasma me te whānuitanga o te pāmahana whakahaere me te hototahi teitei ki te taupū toto.

Ka whakahohehia e te heparin ārai toto te fibrinolysin, me te aukati i te thromboplastin, kātahi ka tutuki te taurite hihiri i waenga i te fibrinogen me te fibrin, me te kore he miro fibrin i te tukanga tirotiro. Ka taea te whakahoki i te nuinga o ngā tohu plasma i roto i ngā haora 6.

Ehara i te mea ko ngā āhuatanga o te heparin konutai anake kei roto i te lithium heparin, engari ka taea hoki te whakamahi i roto i te whakamātautau huānga moroiti me te kore e pā ki te katote konutai. Hei whakatutuki i ngā hiahia maha o te taiwhanga haumanu, ka taea e KANGJIAN te tāpiri i te kirikiri wehenga plasma hei hanga plasma kounga teitei.

Tere whakaterenga: 3500-4000 r/m
Te wā whakapūpū: 3min
Te pāmahana rokiroki e taunakitia ana: 4-25ºC

8. Ngongo PT

E whakamahia ana te ngongo PT mō te whakamātautau toto totoka, ā, e tika ana mō te pūnaha fibrinolytic (PT,TT, APTT me te fibrinogen, me ētahi atu).
Ko te ōwehenga ranunga he 1 wāhanga citrate ki te 9 wāhanga toto. Mā te ōwehenga tika ka taea te whakamana i te whai huatanga o ngā hua whakamātautau, ā, ka karohia hoki te tātaritanga hē.

Nā te mea he iti noa te paitini o te konutai citrate, ka whakamahia hoki hei penapena toto. Me tango te nui o te toto kia tika ai ngā hua whakamātautau. He iti noa te wāhi mate o te ngongo PT he papa rua, ka taea te whakamahi hei aroturuki i te whakamātautau o te v WF, F, ngā mahi a te pereti toto, te rongoā Heparin.