He aha ngā ngongo kohi toto EDTA, ā, me pēhea te whakamahi?

I roto i ngā whakamātautau hauora me te tautuhi me te maimoatanga haumanu,Ngā ngongo kohi toto EDTA, hei taonga matua mō te kohikohi toto, he mea nui ki te whakarite i te pono o ngā tauira me te tika o te whakamātautau. I roto i tēnei tuhinga, ka tātarihia whānuitia e mātou tēnei "kaitiaki ngaro" i roto i te ao hauora mai i ngā āhuatanga o te whakamāramatanga, te whakarōpūtanga tae, te mātāpono ārai toto, te kaupapa whakamātautau me te paerewa whakamahinga.

He aha te meaNgongo kohikohi toto EDTA?

Ko te ngongo kohi toto EDTA he momo ngongo kohi toto korehau kei roto te waikawa Ethylene Diamine Tetraacetic, tōna tote rānei, e whakamahia ana mō te kohi tauira toto me te maimoatanga ārai toto. Ka taea e te EDTA te aukati i te tauhohenga toto toto mā te whakakōpiri i ngā katote konupūmā i roto i te toto, kia noho tonu ai te toto i roto i te āhua wai mō te wā roa, ā, ka whakarato tauira pumau mō ngā whakamātautau toto me te koiora ngota. Ka whakarato tauira pumau mō te whakamātautau toto, te koiora ngota me ētahi atu whakamātautau.

Hei wāhanga nui ongā taonga rongoā e pau anaMe tutuki ngā ngongo kohi toto EDTA ki te paerewa ā-motu o ngā "ipu kohi tauira toto uaua kotahi te whakamahinga" (hei tauira, GB/T 19489-2008) kia pai ai te mahi, kia kore ai e pāwera te toto, kia kore ai e paitini ki te pūtau.

Ngā tae rerekē o ngā ngongo kohi toto EDTA

E ai ki ngā paerewa o te ao whānui (pēnei i ngā aratohu CLSI H3-A6), ko ngā ngongo kohi toto EDTA he mea hipoki ki te papura (EDTA-K2/K3) kikorangi rānei (sodium citrate i konatunatua ki te EDTA) hei wehewehe i te whakamahinga:

Kia mōhio: Tērā pea kua tāpirihia ētahi waitohu ki ētahi atu tae, tirohia ngā tohutohu i mua i te whakamahinga.

Te tikanga ārai toto i roto i ngā ngongo kohi toto EDTA

Mā roto i tōna rōpū carboxyl ngota (-COOH) me ngā iona konupūmā i roto i te toto (Ca²⁺) ka honoa te EDTA hei hanga i tētahi chelate pumau, ā, ka aukati i te whakahohenga o te plasminogen, ka aukati i te tukanga whakapūmau o te fibrinogen ki roto i te fibrin. Ko ngā āhuatanga o tēnei anticoagulation e whai ake nei:

1. te tīmatanga tere o te mahi: ka taea te whakaoti i te rongoā ārai toto i roto i te 1-2 meneti i muri i te kohikohinga toto;

2. te pumau teitei: ka taea te penapena i ngā tauira mō te neke atu i te 48 hāora (ka taea te whakaroa ki te pouaka whakamātao ki te 72 hāora);

3. Whānuitanga o te whakamahinga: he pai mō te nuinga o ngā whakamātautau toto, engari kāore mō ngā whakamātautau toto totoka, whakamātautau mahi pereti rānei (me whakamahi ngongo konutai citrate).

Ngā tūemi whakamātautau matua o te ngongo kohikohi toto EDTA

1. te tātari toto o ia rā: te maha o ngā toto toto mā, ngā tawhā o ngā toto toto whero, te kukū o te hemoglobin, me ētahi atu;

2. te tautuhi i ngā momo toto me te whakataurite: te kimi i te momo toto ABO, te kimi i te tauwehenga Rh;

3. tātaritanga ngota: whakamātautau ira, whakatau i te kawenga huaketo (hei tauira, HIV, HBV);

4. te hemoglobin glycated (HbA1c): te aroturukitanga huka toto mō te wā roa mō te mate huka;

5. te tirotiro i ngā pirinoa toto: te kimi i te Plasmodium, te microfilariae.

Te whakamahi i ngā tikanga me ngā mahi whakatūpato

1. Te tukanga kohikohi:

I muri i te patu huakita i te kiri, me mahi kia rite ki te paerewa o te kohikohinga toto uaua;

I muri tonu i te kohi, hurihia te ngongo kohi toto kia 5-8 ngā wā kia tino konatunatua te rongoā ārai toto ki te toto;

Kaua e rūrū nui (hei ārai i te hemolysis).

2. Te rokiroki me te kawe:

Penapenahia ki te pāmahana rūma (15-25°C), karohia te wera, te huka rānei;

Whakatakotoria poutū i te wā e kawe ana kia kore ai e wetekina te taupoki o te ngongo.

3. ngā āhuatanga ārai:

E hiahiatia ana ngā ngongo konutai citrate mō te Whakapūpū IV (PT, APTT, me ētahi atu);

Me whakamahi he ngongo konutai citrate hei whakamātautau i te mahi a te pereti toto.

Me pēhea te whiriwhiri i te kounga teiteiNgongo kohikohi toto EDTA?

1. Tohu me te tiwhikete: whiriwhiria ngā hua kua paahitia te tiwhikete ISO13485 me te CE. 2;

2. Haumaru rauemi: me mārama te tinana o te ngongo, ā, me kore he toenga kirihou;

3. Te ine tika: me rite te nui o te rongoā ārai toto i tāpirihia ki te paerewa ā-motu (hei tauira, ko te kukū EDTA-K2 he 1.8±0.15mg/mL);

4. Rongonui o te waitohu: Ka tukuna te kaupapa matua ki ngā waitohu rongonui i roto i te mara o ngā taonga hauora hei whakarite i te pumau o te puranga.

Whakamutunga

Hei mema matua otaputapu kohi toto, he pānga tika tā ngā ngongo kohi toto EDTA ki te tika o ngā hua whakamātautau e pā ana ki ō rātou āhuatanga ārai toto. Mā te whakataurite i te whakamahinga o ngā ngongo kohi toto rerekē kua tohua te tae, me te whakakotahi i aua ngongo me ngā tikanga kohi tino pakari, ka taea te whakarato i tētahi pūtake pono mō te tātaritanga haumanu. I te heke mai, me te whanaketanga o te rongoā tika, ka nui ake te mahi a ngā ngongo kohi toto EDTA i roto i te tātaritanga toto, te raupapatanga ira me ētahi atu mara, ā, ka haere tonu te tiaki i te hauora o te tangata.